Recombinant Mouse Clusterin/ApoJ Protein (His Tag)

Specifications

Catalog number

PKSM041216-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Clusterin (CLU) is a secreted protein which belongs to the clusterin family. It is also a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis. Clusterin is an enigmatic glycoprotein with a nearly ubiquitous tissue distribution and an apparent involvement in biological processes ranging from mammary gland involution to neurodegeneration in Alzheimer's disease. Its major form, a heterodimer, is secreted and present in physiological fluids, but truncated forms targeted to the nucleus have also been identified. It is a widely distributed glycoprotein with a wide range of biologic properties. A prominent and defining feature of clusterin is its marked induction in such disease states as glomerulonephritis, cystic renal disease, renal tubular injury, neurodegenerative conditions, atherosclerosis, and myocardial infarction. Upregulation of clusterin mRNA and protein levels detected in diverse disease states and in in vitro systems have led to suggestions that it functions in membrane lipid recycling, in apoptotic cell death, and as a stress-induced secreted chaperone protein, amongst others.

Abbreviation

Clusterin; ApoJ

Synonyms

ApoJ; Apolipoprotein J; Cli; Clusterin; Clustrin; D14Ucla3; SP-40; Sgp-2; Sgp2; Sugp-2; Sulfated glycoprotein 2

Accession Number

Q06890

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu22-Glu448

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

50.4 kDa

Observed Molecular Weight

28-50&70 kDa

Species

Mouse