Recombinant Mouse CD80/B7-1 Protein (His Tag)

Specifications

Catalog number

PKSM041219-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cluster of Differentiation 80, also called B7-1, is a member of cell surface immunoglobulin superfamily which plays key, yet distinct roles in the activation of T cells. Mouse CD80 consists of an extracellular domain (ECD) with twoimmunoglobulin-like domains, transmembrane segment, and cytoplasmic domain. B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA4, constitute one of the dominant co-stimulatory pathways that regulate T- and B- cell responses. CD80 is mostly expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20-100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response.

Abbreviation

CD80; B7-1

Synonyms

Activation B7-1 antigen; B7; CD80; Cd28l; Ly-53; Ly53; MIC17; T-lymphocyte activation antigen CD80; TSA1

UniProt

Q00609

Accession Number

Q00609

Expression System

HEK293 Cells

Tag

C-His

Sequence

Val38-Asn246

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

24.6 kDa

Observed Molecular Weight

38-55 kDa

Species

Mouse