Recombinant Mouse CD16/FCGR3 Protein (His Tag)

Specifications

Catalog number

PKSM041224-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Low affinity immunoglobulin gamma Fc region receptor III (Fc gamma RIII/CD16) is a member of the Ig superfamily. Based on close relationships in their extracellular domains, the Fc gamma Rs have been divided into three classes composing of Fc gamma RI (CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16) . Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. Mouse CD16 is a type I transmembrane protein having two extracellular Ig-like domains consisting of immunoglobulin domain, repeat, signa and transmembrane, transmembrane helix. It is expressed on a variety of myeloid and lymphoid cells and associates with Fc R gamma to deliver an activating signal upon ligand binding. Fcgr3 is IgG binding and activation or inhibition of immune responses such as antibody-dependent cellular cytotoxicity, phagocytosis, cell surface receptor signaling pathway and positive regulation of type I/IIa/III hypersensitivity.

Abbreviation

CD16; FCGR3

Synonyms

CD-antigen 16; CD16; Fc gamma Receptor III; FcRIII; Fcgr3; IgG Fc receptor III; Low affinity immunoglobulin gamma Fc region receptor III

UniProt

P08508

Accession Number

P08508

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala31-Thr215

Applications

BLI

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Recombinant Mouse IgG2a Fc on AMC Biosensor, can bind Recombinant Mouse CD16 with an affinity constant of 0.16 uM as determined in BLI assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

22.0 kDa

Observed Molecular Weight

35-40 kDa

Species

Mouse