Recombinant Mouse CD226/DNAM-1 Protein (His Tag)

Specifications

Catalog number

PKSM041233-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse DNAX accessory molecule-1 (DNAM-1) is a type I transmembrane glycoprotein that belongs to the immunoglobulin superfamily. As an activating receptor, it interacts with the ligands CD155 and CD112, and activates natural killer (NK) cells via its immu-noreceptor tyrosine-based activatory motif (ITAM) . Mature mouse DNAM-1 has extracellular domain (ECD) that contains two Ig-like C2-set domains, and possesses a cytoplasmic region that contains motifs for binding PDZ domains. DNAM-1 is expressed on several lymphoid and myeloid cell types and interacts with CD155/PVR and Nectin-2/CD112. Ligation of DNAM-1 promotes the activation of NK cells, CD8+ T cells, and mast cells, induces dendritic cell maturation, initiates megakaryocyte and activated platelet adhesion to vascular endothelial cells, and stimulates monocyte extravasation. Conversely, it inhibits the formation of osteoclasts. Platelet-endothelium interactions that are mediated by DNAM-1 enable the metastasis of tumor cells to the lung.

Abbreviation

CD226; DNAM-1

Synonyms

BC051526; CD226; CD226 antigen; CD226 molecule; DNAM-1; DNAM1; DNAM1 adhesion glycoprotein; DNAX accessory molecule 1; DNAX accessory molecule-1; PTA1; Pta1; T lineage-specific activation antigen 1 antigen; TLiSA1; platelet and T cell activation antigen 1

Accession Number

Q8K4F0

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu19-Pro254

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.6 kDa

Observed Molecular Weight

35-50 kDa

Species

Mouse