Recombinant Mouse PD-L1/B7-H1/CD274 Protein (His Tag)

Specifications

Catalog number

PKSM041248-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse Programmed cell death 1 ligand 1 (Cd274, PD-L1), is a member of the growing B7 family of immune proteins.It involved in the costimulatory signal essential for T-cell proliferation and IFNG production in a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation by blocking cell cycle progression and cytokine production.B7-H1 has been identified as one of two ligands for programmed death1 (PD1), a member of the CD28 family of immunoreceptors. B7-H1 is constitutively expressed in several organs such as heart, skeletal muscle B7-H1 expression is upregulated in a small fraction of activated T and B cells and a much larger fraction of activated monocytes. The costimulatory function of B7-H1 is critical for enhancing maturation and differentiation of T-cells in lymphoid organs.B7-H1 expression is also induced in dendritic cells and keratinocytes after IFN gamma stimulation. Interaction of B7-H1 with PD1 results in inhibition of TCR-mediated proliferation and cytokine production. The B7-H1:PD1 pathway is involved in the negative regulation of some immune responses and may play an important role in the regulation of peripheral tolerance.

Abbreviation

PD-L1; B7-H1; CD274

Synonyms

B7 homolog 1; B7-H1; B7h1; CD274; PD-L1; PDCD1 ligand 1; Pdcd1l1; Pdcd1lg1; Pdl1; Programmed cell death 1 ligand 1Cd274; programmed cell death 1 ligand 1; programmed death ligand 1

UniProt

Q9EP73

Accession Number

Q9EP73

Expression System

HEK293 Cells

Tag

C-His

Sequence

Phe19-Thr238

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized Anti-Human PDL1 mAb-Fc at 2μg/ml (100 μl/well) can bind Mouse PD-L1-His. The ED50 of Mouse PD-L1-His is 23.35 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

25.6 kDa

Observed Molecular Weight

39-58 kDa

Species

Mouse