Recombinant Mouse NGAL/Lipocalin-2 Protein (His Tag)

Specifications

Catalog number

PKSM041250-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Lipocalin-2, also known as Neutrophil Gelatinase-Associated Lipocalin (NGAL), is a secretory protein of the lipocalin superfamily. Lipocalin-2 contains a signal peptide that enables it to be secreted and form complexes with matrix metalloproteinase-9 (MMP-9) through disulfide bonds. Similar to other lipocalin family members, Lipocalin-2 is involved in diverse cellular processes, including the transport of small hydrophobic molecules, protection of MMP-9 from proteolytic degradation, and cell signaling. Furthermore, Lipocalin-2 can tightly bind to bacterial siderophore through a cell surface receptor, possibly serving as a potent bacteriostatic agent by sequestering iron, regulating innate immunity and protecting kidney epithelial cells from ischemia–reperfusion injury. This protein is mainly expressed in neutrophils and in lower levels in the kidney, prostate, and epithelia of the respiratory and alimentary tracts. Recent evidence also suggests its role as a biomarker for renal injury and inflammation.

Abbreviation

NGAL; Lipocalin-2

Synonyms

LCN2; Lipocalin-2; NGAL; Neutrophil gelatinase-associated lipocalin; SV-40-induced 24P3 protein; Siderocalin LCN2; p25

UniProt

P11672

Accession Number

P11672

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gln21-Asn200

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

21.9 kDa

Observed Molecular Weight

20-28 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Histidine-HCl, 8% Trehalose, 2% Glycine, 50mM NaCl, 0.05% Tween 80, pH6.5.

Species

Mouse