Recombinant Mouse CD74 Protein (His Tag)

Specifications

Catalog number

PKSM041252-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse HLA class II histocompatibility antigen gamma chain (CD74), is a single-pass type II membrane glycoprotein which contains 1 thyroglobulin type-1 domain. Mouse CD74 shares 75% and 88% aa sequence identity with human and rat CD74, respectively. CD74 plays an important role in adaptive immunity, inflammation, and cancer. It plays a critical role in MHC class II antigen processing by stabilizing peptide-free class II alpha/beta heterodimers in a complex soon after their synthesis and directing transport of the complex from the endoplasmic reticulum to compartments where peptide loading of class II takes place.CD74 also associates with CD44 and binds with high affinity to the cytokine MIF, leading to inflammatory leukocyte responses, protection from tissue fibrosis, B cell proliferative and survival signaling, and the up-regulation of angiogenic factors in endometrial stromal cells.

Abbreviation

CD74

Synonyms

CD74 antigen; CD74 molecule; Cluster of Differentiation 74; DHLAG gamma chain of class II antigens; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; HLADG; class II invariant chain; major histocompatibility complex

UniProt

P04441

Accession Number

P04441-2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gln56-Leu215

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.4 kDa

Observed Molecular Weight

25-30 kDa

Species

Mouse