Recombinant Mouse Osteoprotegerin/TNFRSF11B Protein (His Tag)

Specifications

Catalog number

PKSM041265-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Osteoprotegerin (OPG, Tnfrsf11b) is a secreted protein that regulates bone density. OPG is widely expressed and constitutively released as a homodimer by mesenchymal stem cells, fibroblasts and endothelial cells. Regulation of its expression by estrogen, parathyroid hormone and cytokines is complex and changes with age. OPG acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. TRAIL decreases the release of OPG from cells that express it, while OPG inhibits TRAIL-induced apoptosis. Expression of RANK L on the cell surface, and thus its ability to stimulate osteoclastogenesis, is regulated by OPG by intracellular and extracellular mechanisms. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. It may also play a role in preventing arterial calcification.

Abbreviation

Osteoprotegerin; TNFRSF11B

Synonyms

Ocif; Opg; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TR1; Tnfrsf11b; Tumor necrosis factor receptor superfamily member 11B

UniProt

O08712

Accession Number

O08712

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu22-Leu401

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

45.1 kDa

Observed Molecular Weight

50-65 kDa

Species

Mouse