Recombinant Mouse MCPT6/Mast Cell Protease-6 Protein (His Tag)

Specifications

Catalog number

PKSM041266-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tryptase beta-2 (Tpsb2), also known as Mast cell protease 6 (mMCP-6), belongs to the peptidase S1 family and Tryptase subfamily. Tryptase is the major neutral protease present in mast cells and is secreted upon the coupled activation-degranulation response of this cell type. It plays a role in innate immunity. Tpsb2 can be detected primarily in skin during embryogenesis. Tpsb2 can not be detected at early embryonic stages but is abundantly expressed in later stages with a peak at E17.5-E18.5. Tryptase is a homotetramer. The active tetramer is converted to inactive monomers at neutral and acidic pH in the absence of heparin. Low concentrations of inactive monomers become active monomers at pH 6.0 in the presence of heparin. When the concentration of active monomers is higher, they convert to active monomers and then to active tetramers. These monomers are active and functionally distinct from the tetrameric enzyme. In contrast to the hidden active sites in the tetrameric form, the active site of the monomeric form is accessible for macromolecular proteins and inhibitors eg: fibrinogen which is a substrate for the monomeric but not for the tetrameric form. The monomeric form forms a complex with SERPINB6.

Abbreviation

MCPT6

Synonyms

Mast cell protease 6; Tpsb2; Tryptase beta-2; Tryptase-2; mMCP-6

UniProt

P21845

Accession Number

P21845

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala22-Ser276

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

29.3 kDa

Observed Molecular Weight

32-38 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, 1mM EDTA, pH 8.0.

Species

Mouse