Recombinant Mouse Serpin A3N Protein (His Tag)

Specifications

Catalog number

PKSM041279-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Serine protease inhibitor A3N (Serpin A3N) is a serine protease inhibitor that is structurally related to α1 antichymotrypsin encoded by the SERPINA3 gene. Serpin A3N is highly expressed in brain, testis, lung, thymus, and spleen. It is expressed with low levels in bone marrow, kidney and skeletal muscle. Serpin A3N secreted by Sertoli cells may regulate the activity of locally produced Granzyme B. Granzyme B inhibition by Serpin A3N may therefore regulate Granzyme Bmediated killing by cytotoxic lymphocytes, providing a means to disable cellmediated immune responses.

Abbreviation

Serpin A3N

Synonyms

Serine protease inhibitor A3N; Serpin A3N; Serpina3n; Spi2

UniProt

Q91WP6

Accession Number

Q91WP6

Expression System

HEK293 Cells

Tag

C-His

Sequence

Phe21-Lys418

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

45.6 kDa

Observed Molecular Weight

58 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

Species

Mouse