Recombinant Mouse Osteonectin/SPARC Protein (His Tag)

Specifications

Catalog number

PKSM041280-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Secreted protein acidic and rich in cysteine (SPARC, BM-40) protein belongs to the family of secreted matricellular proteins with similar domain structure. Mouse SPARC protein involved an N-terminal acidic region that binds calcium, a follistatin domain containing Kazal-like sequences, and a C-terminal extracellular calcium (EC) binding domain with two EF-hand motifs. SPARC is produced by fibroblasts, capillary endothelial cells, platelets, and macrophages, especially in areas of tissue morphogenesis and remodeling. It appears to regulate cell growth through interactions with the extracellular matrix and cytokines. SPARC is expressed at high levels in tissues undergoing morphogenesis, remodeling and wound repair. The activity of SPARC is to modulate cell-cell and cell-matrix interactions, and its de-adhesive and growth inhibitory properties in non-transformed cells have led to studies to assess its role in cancer.

Abbreviation

Osteonectin; SPARC

Synonyms

AA517111; BM-40; ON; RP23-465I4.1; SPARC; Secreted Protein Acidic and Rich in Cysteine; Sparc

UniProt

P07214

Accession Number

P07214

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala18-Ile302

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

33.6 kDa

Observed Molecular Weight

40 kDa

Species

Mouse