Recombinant Mouse REN1/Renin-1 Protein (His Tag)

Specifications

Catalog number

PKSM041281-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse Renin, also known as Renin-1, is a member of the peptidase A1 amily. Renin is synthesized by the juxtaglomerular cells of the kidney in response to decreased blood pressure and sodium concentration. It cleaves angiotensinogen to generate angiotensin I, which can be further converted by angiotensin converting enzyme (ACE) to angiotensin II. Angiotensin II is the active molecule of the reninangiotensin system that acts by binding to angiotensin receptors type 1 and 2 (AT1 and AT2), and has direct pathophysiological effects on the heart and peripheral vasculature. After secretion, inactive prorenin can be proteolytically activated by trypsin, cathepsin B, or other proteinases.

Abbreviation

REN1; Renin-1

Synonyms

Angiotensin-forming enzyme; Angiotensinogenase; Kidney renin; Ren; Ren-1; Ren-A; Ren1; Ren1c; Ren1d; Renin-1; Rn-1; Rnr

UniProt

P06281

Accession Number

P06281

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu22-Arg402

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43.5 kDa

Observed Molecular Weight

41-60 kDa

Species

Mouse