Recombinant Mouse SFTPD/SP-D Protein (His Tag)

Specifications

Catalog number

PKSM041282-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Pulmonary surfactant-associated protein D (SP-D) is a 43 kDa member of the collectin family of innate immune modulators. Mouse SP-D cDNA encodes a 19 aa signal sequence and a 355 aa mature region with a 25 aa N-terminal linking-region, a 177 aa hydroxyproline and hydroxylysine collagen-like domain, a 46 aa coiled-coil segment, and a 106 aa, C-terminal collectin-like C-type lectin domain . SP-D is found in serum, plasma, broncho-alveolar lavage (BAL) fluid, and amniotic fluid. It also binds SIRP alpha and the calreticulin/CD91 complex on macrophages. SP-D contributes to the lung' s defense against inhaled microorganisms, organic antigens and toxins. It Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. It may participate in the extracellular reorganization or turnover of pulmonary surfactant. It binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.

Abbreviation

SFTPD; SP-D

Synonyms

COLEC7; Collectin 7; Lung surfactant protein D; PSPD; Pulmonary surfactant-associated protein D; SFTP4; SFTPD; SP-D; SP-Dpulmonary surfactant apoprotein; SPD; pulmonary-associated protein D; surfactant; surfactant protein D

UniProt

P50404

Accession Number

P50404

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala20-Phe374

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

36.7 kDa

Observed Molecular Weight

42 kDa

Species

Mouse