Recombinant Mouse Motch A/NOTCH1 Protein (His Tag)

Specifications

Catalog number

PKSM041299-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse Notch1 is a 300 kDa type I transmembrane glycoprotein and it functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Mouse Notch1 is synthesized as a 2531 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1707 aa extracellular domain (ECD) with 36 EGFlike repeats and three Lin12/notch repeats, a 21 aa transmembrane segment and a 785 aa cytoplasmic domain that contains six ankyrin repeats, a glutamine-rich domain and a PEST sequence. Notch1 may play an essential role in postimplantation development, probably in some aspect of cell specification and/or differentiation and may be involved in mesoderm development, somite formation and neurogenesis.

Abbreviation

Motch A; NOTCH1

Synonyms

Mis6; Motch A; Mt14; N1; Neurogenic locus notch homolog protein 1; Notch 1; Tan1; lin-12

UniProt

Q01705

Accession Number

Q01705

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ala18-Gln526

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

54.4 kDa

Observed Molecular Weight

70 kDa

Species

Mouse