Recombinant Mouse Mesothelin/MSLN Protein (His Tag)

Specifications

Catalog number

PKSM041304-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mesothelin is derived from a 70 kDa precursor that also includes Megakaryocyte Potentiating Factor (MPF) .The 70 kDa precursor is expressed on the cell surface where it is cleaved at a dibasic proteolytic site to release the 32 kDa glycosylated MPF. MPF is a cytokine that potentiates IL3 induced megakaryocyte colony formation. The term Mesothelin refers to the 40 kDa glycosylated protein which remains attached to the cell surface via a GPI linkage. Mesothelin is over expressed in several human tumors, including mesothelioma and ovarian and pancreatic adenocarcinoma. The interaction between mesothelin and MUC16 (also known as CA125) may facilitate the implantation and peritoneal spread of tumors by cell adhesion. The region (296-359) consisting of 64 amino acids at the N-terminal of cell surface mesothelin is the functional binding domain for MUC16. Mesothelin is a tumour differentiation antigen that is normally present on the mesothelial cells lining the pleura, peritoneum and pericardium. A soluble form of Mesothelin, with its GPI anchor intact, is released from tumor cells and binds to MMR/CD206 on macrophages.

Abbreviation

Mesothelin; MSLN

Synonyms

CAK1; MSLN ; Mes; Mesothelin; Mpf; Msln

UniProt

Q61468

Accession Number

Q61468

Expression System

HEK293 Cells

Tag

C-His

Sequence

Asp298-Ser600

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

35.0 kDa

Observed Molecular Weight

38-55 kDa

Species

Mouse