Recombinant Mouse SCF/c-Kit Ligand Protein (His Tag)

Specifications

Catalog number

PKSM041311-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SCF/C-kit ligand is the ligand of the tyrosine-kinase receptor encoded by the KIT locus. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. In phase I/II clinical studies administration of the combination of SCF and G-CSF resulted in a two- to threefold increase in cells that express the CD34 antigen compared with G-CSF alone.

Abbreviation

SCF; c-Kit Ligand

Synonyms

FPH2; Hematopoietic growth factor KL; KIT ligand; KITLG; KL-1; Kitl; MGF; MGFSHEP7; Mast cell growth factor; SCF; SFc-Kit ligand; SLF; Stem cell factor; steel factor

UniProt

P20826

Accession Number

P20826

Expression System

HEK293 Cells

Tag

C-His

Sequence

Lys26-Ala189

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.3 kDa

Observed Molecular Weight

19-35 kDa

Species

Mouse