Recombinant Mouse KIRREL1/NEPH1 Protein (His Tag)

Specifications

Catalog number

PKSM041312-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Kin of irregular chiasm-like protein 1 (Kirrel1), also known as Nephrin-like protein 1 (Neph1), belongs to the immunoglobulin superfamily. Kirrel1 plays a significant role in the normal development and function of the glomerular permeability. It is a signaling protein that needs the presence of TEC kinases to fully trans-activate the transcription factor AP-1. The knockout of this gene could result in perinatal lethality accompanied by proteinuria, and effacement of glomerular podocytes. Kirrel1 is abundantly expressed in kidney and specifically expressed in podocytes of kidney glomeruli. Its’ subunit interacts with TJP1/ZO-1 and with NPHS2/podocin (via the C-terminus) and interacts with NPHS1/nephrin (via the Ig-like domains) . This interaction is dependent on KIRREL glycosylation. Kirrel1 also interacts when tyrosine-phosphorylated with GRB2.

Abbreviation

KIRREL1; NEPH1

Synonyms

Kin of IRRE-like protein 1; Kin of irregular chiasm-like protein 1; Kirrel1; Neph1; Nephrin-like protein 1

UniProt

Q80W68

Accession Number

Q80W68

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu48-Leu525

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

53.4 kDa

Observed Molecular Weight

70-85 kDa

Species

Mouse