Recombinant Mouse ICOS Ligand/ICOSL Protein (His Tag)

Specifications

Catalog number

PKSM041331-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mouse ICOS ligand (B7-H2) is an approximately transmembrane glycoprotein in the B7 family of immune regulatory molecules. B7-H2 is expressed on antigen presenting cells such as B cells, macrophages, monocytes, and dendritic cells. It binds to ICOS on activated T cells, leading to both positive and negative effects on immune responses including its own down-regulation. The B7-H2 interaction with ICOS is costimulatory for T cell proliferation as well as the development of B cells, plasma cells, follicular helper T cells and germinal centers. B7-H2 contributes to the development of allergic asthma by enhancing Th2 biased immune responses, limiting Th17 responses, and promoting eosinophilic infiltration into the lung. Its activation of ICOS on Treg limits pulmonary inflammation and airway hyperresponsiveness, promotes the development of inhalational tolerance, and impairs antitumor immunity. In the thyroid, B7-H2 is up-regulated on thyrocytes during inflammation and promotes their proliferation and production of thryoid hormones.

Abbreviation

ICOS Ligand; ICOSL

Synonyms

B7 homolog 2; B7-H2; B7-like protein Gl50; B7H2; B7RP-1; B7RP-1LICOS; B7RP1; CD275; CD275 antigen; GL50; ICOS Ligand; ICOS ligand; ICOS-L; ICOSL; ICOSLG; inducible T-cell co-stimulator ligand

UniProt

Q9JHJ8

Accession Number

Q9JHJ8

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu47-Lys279

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.1 kDa

Observed Molecular Weight

40-60 kDa

Species

Mouse