Recombinant Mouse Complement Factor H/CFH Protein (His Tag)

Specifications

Catalog number

PKSM041346-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Complement factor H (CFH) is a 155 kDa glycoprotein that functions as a cofactor in the inactivation of C3b by factor I. It also increases the rate of dissociation of the C3bBb complex (C3 convertase) and the (C3b) NBB complex (C5 convertase) in the alternative complement pathway. CFH expressed by the liver and secreted in plasma. This recombinant protein corresponds to SCR15-20 which encompass the primary binding sites for heparin and C3b as well as for the peptide hormone adrenomedullin. Within SCR15-20, mouse Factor H shares 60% and 80% amino acid sequence identity with human and rat Factor H, respectively. Dozens of mutations clustered in SCR15-20 are associated with atypical hemolytic uremic syndrome, a disorder characterized by anemia, thrombocytopenia, and renal failure.

Abbreviation

Complement Factor H; CFH

Synonyms

CFH; Complement factor H; Protein beta-1-H; beta-1-H-globulin

UniProt

E9Q8I0

Accession Number

E9Q8I0

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ser875-Val1252

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

43.4 kDa

Observed Molecular Weight

50-75 kDa

Species

Mouse