Recombinant Mouse SynCam/CADM1 Protein (aa 48-388, His Tag)

Specifications

Catalog number

PKSM041349-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cell adhesion molecule 1 (CADM1) is a single-pass type I membrane protein and belongs to the nectin family. It contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. CADM1 acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. CADM1 may contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, it may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. The protein acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly. It may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

Abbreviation

SynCam; CADM1

Synonyms

Cell adhesion molecule 1; IgSF4; Immunoglobulin superfamily member 4; NECL-2; Nectin-like protein 2; SynCAM; Synaptic cell adhesion molecule; TSLC-1; Tumor suppressor in lung cancer 1

UniProt

Q8R5M8

Accession Number

Q8R5M8

Expression System

HEK293 Cells

Tag

C-His

Sequence

Gln48-His388

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

39.2 kDa

Observed Molecular Weight

60-100 kDa

Species

Mouse