Recombinant Mouse Bone Sialoprotein 2/IBSP Protein (His Tag)

Specifications

Catalog number

PKSM041355-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Bone sialoprotein 2 (IBSP) is a monomeric non-collagenous member of the SIBLING family of extracellular matrix proteins. It is principally associated with the early stages of bone mineralization. Mouse IBSP is synthesized as a 324 amino acid (aa) precursor that contains a 16 aa signal sequence and a 308 aa mature region. The mature segment is divided into a basic N-terminus (aa 17 - 62), a central region (aa 63 - 233), and an acidic C-terminus (aa 234 - 317) . IBSP is highly glycosylated, sulfated and phosphorylated. Phosphorylation promotes HAp nucleation, while carbohydrate may regulate cell adhesion. IBSP binds tightly to hydroxyapatite, appears to form an integral part of the mineralized matrix. It is probably important to cell-matrix interaction and promotes Arg-Gly-Asp-dependent cell attachment.

Abbreviation

Bone Sialoprotein 2; IBSP

Synonyms

BNSP; BSP; BSP2; BSPII; Bone sialoprotein; Bone sialoprotein 2; Cell binding sialoprotein; IBSP; Integrin binding sialoprotein; SP II; Sialoprotein II

UniProt

Q61711

Accession Number

AAA21726.1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Phe17-Gln324

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

35.1 kDa

Observed Molecular Weight

71 kDa

Species

Mouse