Recombinant Mouse CD86/B7-2 Protein (Fc Tag)

Specifications

Catalog number

PKSM041356-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

T-lymphocyte activation antigen CD86 (B7-2) is a glycosylated protein in the B7 family. B7 family members are transmembrane cell surface molecules that play important roles in immune activation and the maintenance of immune tolerance. Mouse CD86 shares 59% and 81% aa sequence identity with human and rat CD86, respectively. It contains 1 Ig-like C2-type domainand 1 Ig-like V-type domain. It is highly expressed on activated antigen presenting cells. CD86 involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. It may play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. It is expressed by activated B-lymphocytes and monocytes and promoted by MARCH8 and results in endocytosis and lysosomal degradation.

Abbreviation

CD86; B7-2

Synonyms

Activation B7-2 antigen; B7; B7-2; B7.2; B70; CD86; CLS1; Cd28l2; ETC-1; Early T-cell costimulatory molecule 1; Ly-58; Ly58; MB7; MB7-2; T-lymphocyte activation antigen CD86; TS/A-2

UniProt

P42082

Accession Number

P42082

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Val26-Glu245

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

52.2 kDa

Observed Molecular Weight

68-95 kDa

Species

Mouse