Recombinant Human/Mouse/Rat Irisin/FNDC5 (N-6His)

Specifications

Catalog number

PKSM041407-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Fibronectin type III domain-containing protein 5, the precursor of irisin, is a protein that is encoded by the FNDC5 gene.Human Irisin is synthesized as a 212 amino acid (aa) precursor encoding a type 1 transmembrane protein with a 121 aa extracellular domain (ECD), a 21 aa transmembrane domain, and a 39 aa cytoplasmic domain. The ECD of Irisin contains a fibronectin type III domain and multiple glycosylation sites. The ECD is proteolytically cleaved to release the 112 aa soluble Irisin hormone into circulation.Mature human, mouse share 100% sequence identity.Irisin induces expression of peroxisome proliferatoractivated receptor γ coactivator 1α (PGC1α) and uncoupling protein1 (UCP1), mitochondrialassociated metabolic proteins. Irisin induces the transition of white adipose tissue into more metabolically active beige adipose tissue.Irisin also regulates neuronal cell differentiation and neurite outgrowth in the brain and is involved in the differentiation of osteoblasts.

Abbreviation

Irisin; FNDC5

Synonyms

FNDC5; Fibronectin type III domain-containing protein 5; Fibronectin type III repeat-containing protein 2; Irisin

UniProt

Q8NAU1

Accession Number

Q8NAU1

Expression System

HEK293 Cells

Tag

N-His

Sequence

Asp32-Glu143

Field of Research

Cell Biology, Metabolism

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

13.4 kDa

Observed Molecular Weight

18-28 kDa

Citation 01

https://www.mdpi.com/1879598

Species

Human/Mouse/Rat