Recombinant Mouse ALK-5/TGFBR1 (C-Fc)

Specifications

Catalog number

PKSM041408-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

TGF-beta RI, also called ALK-5, is an approximately 55 kDa type I transmembrane serine/threonine receptor kinase. In the presence of TGF-beta, TGF-beta RI forms a complex with, and is phosphorylated by, TGF-beta RII. Phosphorylated TGF-beta RI can then transiently bind and phosphorylate Smad2 and Smad3. TGF-beta functions as a tumor suppressor by inhibiting the cell cycle in the G1 phase. Administration of TGF-beta is able to protect against mammary tumor development in transgenic mouse models in vivo. Disruption of the TGF-beta/SMAD pathway has been implicated in a variety of human cancers, with the majority of colon and gastric cancers being caused by an inactivating mutation of TGF-beta RII. TGF-beta RI is likely important during development, since mice deficient for TGF-beta RI die at midgestation with severe defects in vascular development of the yolk sac and placenta, and an absence of circulating red blood cells. Furthermore, TGF-beta RI appears to be involved in proper lymphatic network development.

Abbreviation

ALK-5; TGFBR1

Synonyms

53kD; AAT5; ACVRLK4; ALK-5; ALK-5ALK5; LDS1A; LDS2A; SKR4; TGF-bRI; TGF-beta receptor type I; TGFB1R1; TGFBR1; TGFR-1; TGFbetaRI; activin A receptor type II-like kinase; tbetaR-I

UniProt

Q64729

Accession Number

Q64729

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Leu30-Glu125

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

37.6 kDa

Observed Molecular Weight

40-60 kDa

Species

Mouse