Recombinant Mouse G-CSF protein (N-His)

Specifications

Catalog number

PKSM041499-01

Size / count

20 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Granulocyte colony-stimulating factor (G-CSF) is a growth factor and an essential cytokine which belongs to the IL-6 superfamily. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. G-CSF binding to its receptor G-CSF-R which belongs to the cytokine receptor type I family depends on the interaction of alpha-helical motifs of the former and two fibronectin type III as well as an immunoglobulin-like domain of the latter. G-CSF is a cytokine that have been demonstrated to improve cardiac function and perfusion in myocardial infarction. And it was initially evaluated as a stem cell mobilizer and erythropoietin as a cytoprotective agent.

Abbreviation

G-CSF

Synonyms

OPGL; CD254; ODF (Osteoclast Differentiation Factor) ; TNFSF11; TRANCE (TNF-Related Activation-induced Cytokine) ; sRNAK Ligand; soluble Receptor Activator of NF-kB Ligand

UniProt

P09920

Accession Number

P09920

Expression System

E.coli

Tag

N-His

Sequence

Val 31-Ala 208

Applications

Cell culture

Endotoxin

< 0.1 EU per μg of the protein as determined by the LAL method.

Purity

> 98 % as determined by reducing SDS-PAGE.

Bioactivity

50 2 x 107 IU/mg.7

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

19.8 kDa

Observed Molecular Weight

17-25 kDa

Species

Mouse