Recombinant Cynomolgus SIGLEC5/CD170 Protein (His Tag)

Specifications

Catalog number

PKSQ050010-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Sialic acid-binding Ig-like lectin 5 is a protein that in Cynomolgus is encoded by the SIGLEC5 gene, Cynomolgus SIGLEC5 cDNA encodes 551 amino acids (aa) that include a 16 aa signal sequence, a 439aa extracellular domain (ECD) with three Ig-like domains, a transmembrane region and a cytoplasma tail. No Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. Siglec5 to 11 share a high degree of sequence similarity with CD33/Siglec3 both in their extracellular and intracellular regions. Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Binds equally to alpha-2,3-linked and alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface.

Abbreviation

SIGLEC5; CD170

Synonyms

CD170; CD33L2; OB-BP2; OBBP; OBBP2; SIGLEC-5; SIGLEC5; Sialic acid-binding Ig-like lectin 5; Siglec-5

UniProt

A0A0B4J1D1

Accession Number

A0A0B4J1D1

Expression System

HEK293 Cells

Tag

C-His

Sequence

Glu17-Gly435

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

46.8 kDa

Observed Molecular Weight

90 kDa

Species

Cynomolgus macaques