Recombinant Cynomolgus CRTAM/CD355 Protein (Fc Tag)

Specifications

Catalog number

PKSQ050012-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cytotoxic and Regulatory T-Cell Molecule (CRTAM) is a member of Nectin family under the immunoglobulin superfamily that is expressed by activated CD8+ and NK T cells. CRTAM is found in spleen, thymus, small intestine, peripheral blood, and it is highly expressed by Purkinje cells of the cerebellum. CRTAM is a type I transmembrane glycoprotein containing one Ig-like C2-type domain and one Ig-like V-type domain in its extracellular domain, while its cytoplasmic region shows a potential class I PDZ domain. CRTAM is expressed as a homodimer on the cell surface but does not show homotypic binding in trans. The high affinity of CRTAM/IGSF4 adhesion allows CRTAM to disrupt IGSF4 homotypic interactions. IGSF4 and T cell receptor coengagement of CD8+ cells expressiong CRTAM induces increased IFNγ or IL-22 production.

Abbreviation

CRTAM; CD355

Synonyms

CD355; CRTAM; Class-I MHC-Restricted T-Cell-Associated Molecule; Cytotoxic and Regulatory T-Cell Molecule

UniProt

A0A2K5TKL4

Accession Number

A0A2K5TKL4

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Ser18-Gly287

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

57.1 kDa

Observed Molecular Weight

80-90 kDa

Species

Cynomolgus macaques