Recombinant Cynomolgus TIM-3/HAVCR2 Protein (Fc Tag)

Specifications

Catalog number

PKSQ050022-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

T cell immunoglobulin and mucin domain 3 is a member of the TIM family of immune regulating molecules. Mature cynomolgus TIM3 consists of a 182 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 78 aa cytoplasmic tail. TIM3 is up-regulated on several populations of activated myeloid cells (macrophage, monocyte, dendritic cell, microglia, mast cell) and T cells (Th1, CD8+, NK, Treg) . Its binding to Galectin9 induces a range of immunosuppressive functions which enhance immune tolerance and inhibit anti-tumor immunity. TIM3 ligation attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. TIM3 interactions with Galectin-9 can trigger immune stimulatory effects, such as the coactivation of NK cell cytotoxicity.

Abbreviation

TIM-3; HAVCR2

Synonyms

HAVCR2; T cell immunoglobulin and mucin domain3; TIM3; Tim-3

UniProt

G7P6Q7

Accession Number

G7P6Q7

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Ser22-Arg201

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

46.3 kDa

Observed Molecular Weight

55-75 kDa

Species

Cynomolgus macaques