Recombinant Cynomolgus PD-1/CD279/PDCD1 Protein (Fc Tag)

Specifications

Catalog number

PKSQ050027-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Programmed cell death protein 1 (PDCD1) is a single-pass type I membrane protein and contains 1 Ig-like V-type domain. PD-1 is a member of the extended CD28/CTLA-4 family of T cell regulators. PDCD1 inhibits the T-cell proliferation and production of related cytokines including IL-1, IL-4, IL-10 and IFN-γ by suppressing the activation and transduction of PI3K/AKT pathway. In addition, coligation of PDCD1 inhibits BCR-mediating signal by dephosphorylating key signal transducer. PDCD1 has been suggested to be involved in lymphocyte clonal selection and peripheral tolerance, and thus contributes to the prevention of autoimmune diseases. As a cell surface molecule, PDCD1 regulates the adaptive immune response. Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function.

Abbreviation

PD-1; CD279; PDCD1

Synonyms

PD-1; PD1; Programmed cell death 1; Programmed cell death protein 1

UniProt

B0LAJ3

Accession Number

B0LAJ3

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Leu25-Gln167

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

42.9 kDa

Observed Molecular Weight

60-75 kDa

Species

Cynomolgus macaques