Recombinant Cynomolgus CD3e/CD3ε Protein (Fc Tag)

Specifications

Catalog number

PKSQ050030-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

T-cell surface glycoprotein CD3 epsilon chain, also known as CD3E, is a single-pass type I membrane protein. CD3E contains 1 Ig-like (immunoglobulin-like) domain and 1 ITAM domain. CD3E, together with CD3-gamma, CD3-delta and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. The CD3 epsilon subunit of the T cell receptor (TCR) complex contains two defined signaling domains, a proline-rich sequence and an immune tyrosine activation motifs (ITAMs), and this complex undergoes a conformational change upon ligand binding that is thought to be important for the activation of T cells. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. This complex is critical for T-cell development and function, and represents one of the most complex transmembrane receptors. CD3E plays an essential role in T-cell development, and defects in CD3E gene cause severe immunodeficiency. Homozygous mutations in CD3D and CD3E genes lead to a complete block in T-cell development and thus to an early-onset severe combined immunodeficiency phenotype.

Abbreviation

CD3e; CD3ε

Synonyms

CD3 epsilon; CD3-epsilon; CD3e; CD3e antigen; CD3e molecule; FLJ18683; T-cell antigen receptor complex; T-cell surfa; T-cell surface antigen T3/Leu-4 epsilon chain; T3E; epsilon (CD3-TCR complex) ; epsilon polypeptide (TiT3 complex) ; epsilon subunit of T3

UniProt

Q95LI5

Accession Number

Q95LI5

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Gln22-Asp117

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

38 kDa

Observed Molecular Weight

38-55 kDa

Species

Cynomolgus macaques