Recombinant Cynomolgus B7-H3/CD276 Protein (Fc Tag)

Specifications

Catalog number

PKSQ050036-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD276, also known as B7-H3, is a member of the B7 superfamily with signature IgV and IgG regions in extracellular domains. It is a type I transmembrane protein and shares 20–27% amino acid identity with other B7 family members. B7-H3 is involved in the activation of T lymphocytes, and regulates murine bone formation. It is also reported that B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is expressed on T-cells, natural killer cells, and antigen presenting cells, as well as some non-immune cells, such as osteoblasts, fibroblasts, fibroblast-like synoviocytes and epithelial cells. High expression of B7-H3 in tumor vasculature also correlates with poor survival in patients, suggesting that it may play a role in tumor cell migration.

Abbreviation

B7-H3; CD276

Synonyms

B7 homolog 3; B7-H3; CD276; CD276 antigen

UniProt

A0A7N9CYV2

Accession Number

XP_015308534

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Leu29-Glu465

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

74.0 kDa

Observed Molecular Weight

100-130 kDa

Species

Cynomolgus macaques