Recombinant Cynomolgus PD-L1/B7-H1/CD274 Protein (Fc Tag)

Specifications

Catalog number

PKSQ050046-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

CD274, also known as B7-H1 or programmed death ligand 1 (PD-L1), is a 40 kD type I transmembrane protein and a member of the B7 family within the immunoglobulin receptor superfamily. Programmed death-1 ligand-1 (PD-L1, CD274, B7-H1) has been identified as the ligand for the immunoinhibitory receptor programmed death-1 (PD1/PDCD1) and has been demonstrated to play a role in the regulation of immune responses and peripheral tolerance. By binding to PD1 on activated T-cells and B-cells, PD-L1 may inhibit ongoing T-cell responses by inducing apoptosis and arresting cell-cycle progression. Accordingly, it leads to growth of immunogenic tumor growth by increasing apoptosis of antigen specific T cells and may contribute to immune evasion by cancers. PD-L1 thus is regarded as promising therapeutic target for human autoimmune disease and malignant cancers.

Abbreviation

PD-L1; B7-H1; CD274

Synonyms

B7-H; B7-H1; B7H1; B7H1PDCD1L1; CD274; CD274 antigenMGC142294; CD274 molecule; PD-L1; PD-L1B7 homolog 1; PDCD1L1; PDCD1LG1; PDL1; PDL1PDCD1 ligand 1; Programmed death ligand 1; programmed cell death 1 ligand 1

UniProt

G7PSE7

Accession Number

G7PSE7

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Phe19-Thr239

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

52.4 kDa

Observed Molecular Weight

73 kDa

Species

Cynomolgus macaques