Recombinant E.coli Tryptophan Synthase β Chain/Trp B Protein (His Tag)

Specifications

Catalog number

PKSQ050055-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tryptophan synthase is an enzyme that catalyzes the final two steps in the biosynthesis of tryptophan. It is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from animals such as humans. Tryptophan synthase typically exists as an α-ββ-α complex.The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3-phosphate: L-serine + 1-C- (indol-3-yl) glycerol 3-phosphate = L-tryptophan + D-glyceraldehyde 3-phosphate + H2O.The beta subunits catalyze the irreversible condensation of indole and serine to form tryptophan in a pyridoxal phosphate (PLP) dependent reaction. Their assembly into a complex leads to structural changes in both subunits resulting in reciprocal activation.

Abbreviation

Tryptophan Synthase β Chain; Trp B

Synonyms

Tryptophan synthase beta chain; trpB

UniProt

P0A879

Accession Number

P0A879

Expression System

E.coli

Tag

N-His

Sequence

Thr2-Ile397

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

43.8 kDa

Observed Molecular Weight

38-48 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 8% Sucrose, 0.05% Tween 80, pH 8.5.

Species

E.coli