Recombinant E.coli Tryptophan Synthase Protein (His Tag)

Specifications

Catalog number

PKSQ050057-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Tryptophan synthase is a multienzyme α2β2 complex composed of two protein subunit. Tryptophan synthase catalyzes the last two steps in the synthesis of L-tryptophan (L-Trp) . The α-subunit catalyzes cleavage of 3-indole-d-glycerol 3′-phosphate (IGP) to give indole and D-glyceraldehyde 3′-phosphate (G3P) . Indole is then transferred through a 25-hydrophobic tunnel to the β-subunit. The β2 subunit contains pyridoxal 5'-phosphate and catalyzes several pyridoxal 5'-phosphate-dependent reactions, including/3-elimination reactions 6 and a thiol-dependent transamination reaction. This enzyme is commonly found in Eubacteria, Archaebacteria, Protista, Fungi, and Plantae, but is absent from Animalia. As humans do not have tryptophan synthase, this enzyme has been explored as a potential drug target.

Abbreviation

Tryptophan Synthase

Synonyms

Tryptophan synthase; Tryptophan synthetase

UniProt

P0A877; P0A879

Accession Number

P0A877&P0A879

Expression System

E.coli

Tag

N-His

Sequence

Met1-Ser268&Thr2-Ile397

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

28.7&43.8 kDa

Observed Molecular Weight

28&40-50 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Species

E.coli