Recombinant E.coli Beta-galactosidase Protein

Specifications

Catalog number

PKSQ050060-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Β-galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between a galactose and its organic moiety. It may also cleave fucosides and arabinosides but with much lower efficiency. β-galactosides include carbohydrates containing galactose where the glycosidic bond lies above the galactose molecule. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins. It is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. In E. coli, the gene of β-galactosidase, the lacZ gene, is present as part of the inducible system lac operon which is activated in the presence of lactose when glucose level is low. β-galactosidase is important for organisms as it is a key provider in the production of energy and a source of carbons through the break down of lactose to galactose and glucose.

Abbreviation

Beta-galactosidase

Synonyms

Beta-gal; Beta-galactosidase; Lactase; lacZ

UniProt

P00722

Accession Number

P00722

Expression System

E.coli

Tag

None

Sequence

Met1-Lys1024 (12-41AA deletion)

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

112.9 kDa

Observed Molecular Weight

115 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Species

E.coli