Recombinant Klebsiella pneumoniae NEO Protein

Specifications

Catalog number

PKSQ050062-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Aminoglycoside 3'-phosphotransferase (APH (3') ), also known as aminoglycoside kinase, is an aminoglycoside-modifying enzyme and widely presented in resistant bacteria. These ATP-dependent enzymes phosphorylate the 3'-hydroxyl of a variety of aminoglycosides including kanamycins, neomycins, paromomycins, neamine, ribostamycin, geneticin, and paromamine. These phosphorylated aminoglycosides fail to bind to their respective ribosomal binding sites with high affinity; hence resistance is conferred to the drugs that are phosphorylated. APH (3') is primarily found in certain species of gram-positive bacteria.

Abbreviation

NEO

Synonyms

APH (3') -II; APH (3') II; Aminoglycoside 3'-phosphotransferase; Kanamycin kinase type II; Neomycin-kanamycin phosphotransferase type II; neo

UniProt

P00552

Accession Number

P00552

Expression System

E.coli

Tag

None

Sequence

Met1-Phe264

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

29 kDa

Observed Molecular Weight

26-30 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 6%Trehalose, 4%Mannitol, 0.05%Tween 80, PH8.0.

Species

Klebsiella pneumoniae