Recombinant Rhesus macaque HVEM/TNFRSF14/CD270 Protein (Fc Tag)

Specifications

Catalog number

PKSQ050075-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Herpesvirus entry mediator (HVEM) is a type I membrane protein in the TNF receptor superfamily, and it can both promote and inhibit T cell activity. HVEM is highly expressed on naïve CD4+ T cells, CD8+ T memory cells, regulatory T cells, dendritic cells, monocytes, and neutrophils. It functions as a receptor for BTLA, CD160, LIGHT/TNFSF14, and Lymphotoxin-alpha. Ligation of HVEM by LIGHT triggers T cell, monocyte, and neutrophil activation and contributes to Th1 inflammation and cardiac allograft rejection. In contrast, HVEM binding to CD160 or BTLA suppresses T cell and dendritic cell activation and dampens intestinal inflammation. HVEM enhances the development of CD8+ T cell memory and Treg function. It is additionally expressed on intestinal epithelial cells, where its binding by intraepithelial lymphocyte (IEL) expressed CD160 promotes epitheilal integrity and host defense. The herpesvirus envelope glycoprotein gD, which binds HVEM to initiate membrane fusion, can antagonize both BTLA and LIGHT binding.

Abbreviation

HVEM; TNFRSF14; CD270

Synonyms

CD270; HVEA; HVEM; Herpes Virus Entry Mediator A; Herpesvirus Entry Mediator A; HveA; TNFRSF14; TR2; Tumor Necrosis Factor Receptor Superfamily Member 14; Tumor Necrosis Factor Receptor-Like 2

UniProt

A0A2K5W0R1

Accession Number

XP_005545061.1

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Pro37-Val203

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

44.7 kDa

Observed Molecular Weight

60 kDa

Species

Rhesus macaque