Recombinant S. cerevisiae TIM14 Protein

Specifications

Catalog number

PKSQ050085-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Mitochondrial import inner membrane translocase subunit TIM14 (TIM14) is an essential component of the PAM complex. PAM complex is required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. In the complex, TIM14 is required to stimulate activity of mtHSP70 (SSC1) . TIM14 belongs to the DnaJ family, which has been involved in Hsp40/Hsp70 chaperone systems. As a mitochondrial chaperone, TIM14 functions as part of the TIM23 complex import motor to facilitate the import of nuclear-encoded proteins into the mitochondria. TIM14 also complexes with prohibitin complexes to regulate mitochondrial morphogenesis, and has been implicated in dilated cardiomyopathy with ataxia.

Abbreviation

TIM14

Synonyms

Mitochondrial import inner membrane translocase subunit TIM14; PAM18; Presequencetranslocated-associated motor subunit PAM18; TIM14

UniProt

Q07914

Accession Number

Q07914

Expression System

E.coli

Tag

None

Sequence

Phe99-Lys168

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

7.9 kDa

Observed Molecular Weight

9 kDa

Species

S. cerevisiae