Recombinant Streptomyces hygroscopicus Bar Protein

Specifications

Catalog number

PKSQ050087-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Phosphinothricin N-acetyltransferase (PAT) is an enzyme that acetylates the free NH2 group of L-phosphinothricin (L-PPT) in the presence of acetyl-CoA as a co-substrate. It is highly specific for L-PPT and does not acetylate other L-amino acids or structurally similar molecules. L-PPT is a glutamate analog that can inhibit glutamine synthetase activity in plants, resulting in the accumulation of ammonia to toxic levels and impairment of photosynthesis. The introduction of a PAT gene into a plant genome can confer resistance to glufosinate herbicide during post-emergent applications.

Abbreviation

Bar

Synonyms

PPT N-acetyltransferase; Phosphinothricin N-acetyltransferase; Phosphinothricin-resistance protein; bar

UniProt

P16426

Accession Number

P16426

Expression System

E.coli

Tag

None

Sequence

Met1-Ile183

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

20.6 kDa

Observed Molecular Weight

18-20 kDa

Species

Streptomyces hygroscopicus