Recombinant Thermobifida fusca Cutinase Protein (His Tag)

Specifications

Catalog number

PKSQ050088-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Cutinase belongs to the family of hydrolases, specifically those acting on carboxylic ester bonds. The systematic name of this enzyme class is cutin hydrolase. Cutinase is a serine esterase containing the classical Ser, His, Asp triad of serine hydrolases. The protein belongs to the alpha-beta class, with a central beta-sheet of 5 parallel strands covered by 5 helices on either side of the sheet. Cutin monomers released from the cuticle by small amounts of cutinase on fungal spore surfaces can greatly increase the amount of cutinase secreted by the spore. The active site cleft is partly covered by 2 thin bridges formed by amino acid side chains, by contrast with the hydrophobic lid possessed by other lipases. The protein also contains 2 disulfide bridges, which are essential for activity, their cleavage resulting in complete loss of enzymatic activity.

Abbreviation

Cutinase; cut2

Synonyms

Cutinase

UniProt

E5BBQ3

Accession Number

E5BBQ3

Expression System

E.coli

Tag

C-His

Sequence

Ala1-Phe261

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

29.5 kDa

Observed Molecular Weight

28-30 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM HAc-NaAc, 50% Glycerol, 5% Mannitol, 0.02% Tween 80, pH4.5.

Species

Thermobifida fusca