Recombinant Vaccinia virus Soluble Interferon α/β Receptor B18 Protein (His Tag)

Specifications

Catalog number

PKSQ050089-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

B18R, also known as B19R in the Copenhagen strain of Vaccinia, is a type I interferon (IFN) -binding protein encoded by the B18R open reading frame in the Western Reserve strain of vaccinia virus. B18R exists in two forms, soluble and membrane-bound form and also has a broad species specificity. B18R has high affinity for human IFN-alpha and also binds rabbit, bovine, rat, pig, and mouse IFN-alpha and IFN-beta. Secreted B18R binds to uninfected and infected cells. B18R presents at the cell surface and protects cells from the antiviral state. It has shown that binding of soluble recombinant B18R protects cultured cells from IFN and allows vaccinia virus replication.

Abbreviation

Interferon αβ Receptor B18

Synonyms

Soluble interferon alpha/beta receptor B18; VACWR200

UniProt

P25213

Accession Number

P25213

Expression System

HEK293 Cells

Tag

C-His

Sequence

His20-Glu351

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

39.2 kDa

Observed Molecular Weight

50-65 kDa

Species

Vaccinia virus