Recombinant Cynomolgus FGL1 (C-6His)

Specifications

Catalog number

PKSQ050095-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Hepassocin, also known as hepatocyte-derived fibrinogen-related protein 1 (HFREP-1), is a liver-specific secreted protein belonging to the fibronogen superfamily, whose members share a fibrinogen domain at their C-termini. It is secreted by the liver and functions as a mitogen for hepatocytes. Hepassocin may play a role in the development of hepatocellular carcinomas. Hepassocin is a disulfide-linked homodimeric protein with a C-terminal fibrinogen domain. It is reported that it is a major immune inhibitory ligand of LAG-3 .

Abbreviation

FGL1

Synonyms

425O18-1; Angiopoietin-like protein 4; Angiopoietin-related protein 4; Angptl4; Farp; Fasting-induced adipose factor; Fiaf; HFARP; Hepatic fibrinogen/angiopoietin-related protein; Ng27; Secreted protein Bk89

UniProt

A0A2K5X990

Accession Number

A0A2K5X990

Expression System

HEK293 Cells

Tag

C-His

Sequence

Leu23-Ile312

Applications

BLI

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Cynomolgus LAG-3-Fc (Cat#PKSQ050015) on Protein A Biosensor, can bind Cynomolgus FGL1-His (Cat#PKSQ050095) with an affinity constant of 28.6nM as determined in BLI assay.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

34.8 kDa

Observed Molecular Weight

35 kDa

Formulation

Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 10% Trehalose, 300mM NaCl, 50% Glycerol, 0.05% Tween 80, pH 8.5.

Species

Cynomolgus macaques