Recombinant Cynomolgus Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-6His)

Specifications

Catalog number

PKSQ050096-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO--terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.

Abbreviation

Siglec-15; CD33L3

Synonyms

425O18-1; Angiopoietin-like protein 4; Angiopoietin-related protein 4; Angptl4; Farp; Fasting-induced adipose factor; Fiaf; HFARP; Hepatic fibrinogen/angiopoietin-related protein; Ng27; Secreted protein Bk89

UniProt

A0A2K5UY47

Accession Number

A0A2K5UY47

Expression System

HEK293 Cells

Tag

C-His

Sequence

Phe20-Thr263

Applications

BLI

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Loaded Anti-Human Siglec15 mAb-mFc on AMQ Biosensor, can bind Cynomolgus Siglec-15-His with an affinity constant of 0.30 nM as determined in BLI assay.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

27.1 kDa

Observed Molecular Weight

30-40 kDa

Species

Cynomolgus macaques