Recombinant Rhesus Macaque SLAM Family Member 7/SLAMF7/CD319/CRACC (C-6His)

Specifications

Catalog number

PKSQ050108-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

SLAMF7 is a single-pass type I membrane protein and contains 1 Ig-like C2-type (immunoglobulin-like) domain. SLAMF7 is expressed in NK cells, activated B-cells, NK-cell line but not in promyelocytic, B-cell lines, or T-cell lines. Although the cytoplasmic domain of CS1 contains immunoreceptor tyrosine-based switch motifs (ITSM), which enables to recruite signaling lymphocyte activation molecule (SLAM) -associated protein (SAP/SH2D1A), it activates NK cells in the absence of a functional SAP. SLAMF7 positively regulated natural killer cell functions by a mechanism dependent on the adaptor EAT-2 but not the related adaptor SAP. However, in the absence of EAT-2, CRACC potently inhibited natural killer cell function. It was also inhibitory in T cells, which are typically devoid of EAT-2. Thus, SLAMF7 can exert activating or inhibitory influences on cells of the immune system depending on cellular context and the availability of effector proteins.

Abbreviation

Macaque

Synonyms

CD2 Subset 1; CD2-Like Receptor-Activating Cytotoxic Cells; CD319; CRACC; CS1; Membrane Protein FOAP-12; Novel Ly9; Protein 19A; SLAM Family Member 7; SLAMF7

UniProt

F7HQ72

Accession Number

F7HQ72

Expression System

HEK293 Cells

Tag

C-His

Sequence

Ser23-Met226

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

23.3 kDa

Observed Molecular Weight

30-50 kDa

Species

Rhesus Macaque