Recombinant Rhesus Macaque Angiotensin-Converting Enzyme 2/ACE-2 (C-Fc)

Specifications

Catalog number

PKSQ050120-01

Size / count

10 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Angiotensin-Converting Enzyme 2 (ACE-2) is an integral membrane protein and a zinc metalloprotease of the ACE family, the ACE family includes somatic and germinal ACE. ACE-2 cleaves angiotensins I and II as a carboxypeptidase, ACE-2 converts angiotensin I to angiotensin 1-9, and angiotensin II to angiotensin 1-7. ACE-2 is also able to hydrolyze apelin-13 and dynorphin-13 with high efficiency. ACE-2 can be high expressed in testis, kidney and heart, in colon, small intestine and ovary at moderate levels. Captopril and lisinopril as the classical ACE inhibitor don’t inhibit ACE-2 activity. ACE-2 may play an important role in regulating the heart function.

Abbreviation

ACE2; ACE-2

Synonyms

ACE-Related Carboxypeptidase; ACE2; ACEH; Angiotensin-Converting Enzyme 2; Angiotensin-Converting Enzyme Homolog; Metalloprotease MPROT15

Accession Number

ACI04564.1

Expression System

HEK293 Cells

Tag

C-Fc

Sequence

Gln18-Val739

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized 2019-nCoV S Protein RBD-SD1-mFc (Cat#PKSR030476) at 2μg/ml (100 μl/well) can bind Rhesus Macaque ACE-2-Fc (Cat#PKSQ050120) . The ED50 of Recombinant Rhesus Macaque ACE-2-His (Cat#PKSQ050120) is 96 ng/ml.

Reconstitution

Not Applicable

Shipping Conditions

This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Storage Conditions

Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Calculated Molecular Weight

110.6 kDa

Observed Molecular Weight

120-140 kDa

Formulation

Supplied as a 0.2 μm filtered solution of PBS, pH7.4.

Species

Rhesus Macaque