Recombinant Rat B7-H6/NCR3LG1 Protein (ECD, Fc Tag)

Specifications

Catalog number

PKSR030136

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Natural cytotoxicity triggering receptor 3 ligand 1 (B7-H6) is a glycosylated member of the B7 family of immune costimulatory proteins. Mature human B7-H6 consists of a 238 amino acid (aa) extracellular domain (ECD) that contains one Ig-like V domain and one Ig-like C1 domain, a 21 aa transmembrane segment, and a 171 aa cytoplasmic domain that contains one ITIM, one SH2, and one SH3 motif. Both of the Ig-like domains carry N-linked glycosylation. The Ig-like V domain mediates 1:1 stoichiometric binding of B7-H6 to NKp30 expressed on NK cells. It does not show binding to NKp44, NKp46, or NKG2D. Ligation of NKp30 by B7-H6 induces NK cell activation and target cell cytolysis. B7-H6 is expressed on a wide range of hematopoietic, carcinoma, and melanoma tumor cells, which is consistent with the detection of NKp30 binding sites on many tumors.

Synonyms

B7H6

Accession Number

XP_006223356.1

Expression System

HEK293 Cells

Tag

C-hFc

Sequence

Met1-Ser308

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized rat NCR3-His at 10μg/mL (100μL/well) can bind rat B7-H6-Fc, the EC50 of rat B7-H6 -Fc is 1-20ng/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

60.4 kDa

Species

Rat