Recombinant Rat PDGFRB/CD140b Protein (His Tag)

Specifications

Catalog number

PKSR030212

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD140b, also known as PDGFRB, is a member of the CD system. CD140b is a cell surface tyrosine kinase receptor essencial for development interacting with the platelet-derived growth factors (PDGFs) which serves as mitogens for mesenchymal cells. CD140b can bind with platelet-derived growth factor (PDGF) -B, that are secreted by tumors and phosphorylation of PDGFR-β was correlated with depth of cancer invasion at statistically significant level.

Synonyms

PDGFR-1; PDGFRB; Pdgfr; Pdgfr1

UniProt

Q05030

Accession Number

Q05030

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Lys530

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized rat PDGFRB-His at 10 μg/ml (100 μl/well) can bind Cynomolgus PDGFB, The EC50 of Cynomolgus PDGFB is 3-7 ng/ml.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

57.6 kDa

Observed Molecular Weight

92 kDa

Species

Rat