Recombinant Rat Galectin-8/LGALS8 Protein (GST Tag)

Specifications

Catalog number

PKSR030300

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

The Galectin family of proteins, with specificity for Nacetyllactosaminecontaining glycoproteins, consists of beta-galactoside binding lectins containing homologous carbohydrate recognition domains (CRDs) . They also possess hemagglutination activity, which is attributable to their bivalent carbohydrate binding properties. Galectins are active both intracellularly and extracellularly. Although they are localized primarily in the cytoplasm and lack a classical signal peptide, galectins can also be secreted by one or more unidentified, non-classical, secretory pathways. They have diverse effects on many cellular functions including adhesion, migration, polarity, chemotaxis, proliferation, apoptosis, and differentiation. Galectins may therefore play a key role in many pathological states, including autoimmune diseases, allergic reactions, inflammation, tumor cell metastasis, atherosclerosis, and diabetic complications. The galectins have been classified into the prototype galectins (1, 2, 5, 7, 10, 11, 13, 14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins (Galectin3) containing one CRD linked to a nonlectin domain, and the tandemrepeat Galectins (4, 6, 8, 9, 12) consisting of two CRDs joined by a linker peptide.Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified nonclassical secretory pathways, galectins can also be secreted to function extracellularly.

Synonyms

30 kDa S-type lectin; RL-30; gal-8; galactose binding; galectin-8; lectin; soluble 8

UniProt

Q6IN24

Accession Number

NP_446314.2

Expression System

E.coli

Tag

N-GST

Sequence

Met 1-Trp 316

Applications

Cell culture

Endotoxin

Please contact us for more information.

Purity

> 90 % as determined by reducing SDS-PAGE.

Bioactivity

Measured by its ability to agglutinate human red blood cells. The ED50 for this effect is typically 0.05-0.3 μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

63.2 kDa

Observed Molecular Weight

55 kDa

Species

Rat