Recombinant Rat Interleukin-25/IL-25 Protein (Fc Tag)

Specifications

Catalog number

PKSR030327

Size / count

50 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-25 (IL-25) is a cytokine that shares sequence similarity with interleukin 17. This cytokine can induce NF-kappaB activation, and stimulate the production of interleukin 8. Both this cytokine and interleukin 17B are ligands for the cytokine receptor IL17BR. IL-25 is a member of the IL-17 family of cytokines. However, unlike the other members of this family, IL-25 promotes T helper (Th) 2 responses. IL-25 also regulates the development of autoimmune inflammation mediated by IL-17– producing T cells. IL-25 and IL-17, being members of the same cytokine family, play opposing roles in the pathogenesis of organ-specific autoimmunity. IL-25 promotes cell expansion and Th2 cytokine production when Th2 central memory cells are stimulated with thymic stromal lymphopoietin (TSLP) – activated dendritic cells (DCs), homeostatic cytokines, or T cell receptor for antigen triggering. Elevated expression of IL-25 and IL-25R transcripts was observed in asthmatic lung tissues and atopic dermatitis skin lesions, linking their possible roles with exacerbated allergic disorders. A plausible explanation that IL-25 produced by innate effector eosinophils and basophils may augment the allergic inflammation by enhancing the maintenance and functions of adaptive Th2 memory cells had been provided.

Abbreviation

Interleukin-25/IL-25

Synonyms

UNQ; Interleukin; IL-17E; IL-25; Interleukin-17E; Interleukin-25; IL17E; IL25; UNQ3120; PRO10272

UniProt

D3ZLB1

Accession Number

D3ZLB1

Expression System

HEK293 Cells

Tag

N-hFc

Sequence

Val17-Ala169

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 85 % as determined by reducing SDS-PAGE.

Bioactivity

Immobilized mouse IL17BR-His at 10 μg/mL (100 μl/well) can bind rat Fc-IL25.The EC50 of rat Fc-IL25 is 0.12-0.27 μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

46.1 kDa

Observed Molecular Weight

49 kDa

Species

Rat