Recombinant Rat IL17RA Protein (His Tag)

Specifications

Catalog number

PKSR030331

Size / count

100 µg

Dry ice

No

Hazardous

No

Discontinued

No

Background

Interleukin-17 receptor (IL-17R), also known as Interleukin-17 receptor A (IL-17RA) and CD217 antigen (CD217), is a cytokine receptor which binds interleukin 17. IL-17R/IL-17RA (CD217) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. IL-17R/IL-17RA (CD217) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor IL-17RA play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure. Defects in IL-17R/IL-17RA (CD217) are the cause of familial candidiasis type 5 (CANDF5) . CANDF5 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans.

Abbreviation

IL17RA

Synonyms

IMD; MGC; CTLA; CANDF; CDw; IL17RA; CANDF5; CD217; CDw217; IL-17RA; IL17R; IMD51; hIL-17R; CD127; CD217 antigen; CDW127; CTLA8; HIL 17R; hIL17R; I17RA; IL 17 receptor; IL 17 receptor A; IL 17RA; IL17; IL-17 R alpha; IL-17 receptor A; IL17A receptor; IL-17R; IL17R-A; IL17-RA; IL-17R-alpha; IL7RA; ILRA; Interleukin 17 receptor A; Interleukin-17 receptor A; MGC10262; CDw217; Interleukin-17 receptor A

Accession Number

NP_001101353.2

Expression System

HEK293 Cells

Tag

C-His

Sequence

Met1-Pro320

Applications

ELISA

Endotoxin

< 1.0 EU per μg of the protein as determined by the LAL method.

Purity

> 95 % as determined by reducing SDS-PAGE.

Bioactivity

1. Immobilized rat IL17RA-His at 10 μg/ml (100 μl/well) can bind biotinylated human IL17A, The EC50 of biotinylated human IL17A is 5-11.8 ng/ml. 2. Immobilized mouse IL17a at 10 μg/ml (100 μl/well) can bind rat IL17RA-His, The EC50 of rat IL17RA-His is 0.44-1.02 μg/mL.

Reconstitution

Please refer to the printed manual for detailed information.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

34.1 kDa

Observed Molecular Weight

52-58 kDa

Species

Rat